#J20 STEREOLOGY FREE#

We suggest that oligomeric AОІ directly induces neurogenesis in vivo as has been demonstrated in vitro. The increase in neurogenesis correlated with detectable levels of oligomeric AОІ, measured by ELISA and western blot. Using immunohistochemistry, we show increased proliferation and neuronal differentiation in the hippocampi of 3 month-old J20 mice that reverted when animals became older.

In order to clarify the contradicting data reported in vivo, we investigated NSC proliferation and neuronal differentiation in the hippocampi of J20 mice at a broader range of ages. We have previously reported that a specific aggregation of AОІ has neurogenic potential on neural stem cells (NSC) in vitro. Only one study demonstrates increased neurogenesis in the hippocampus of APP-overexpressing J20 mice, and suggests it is a compensatory effect due to a subtle AОІ-induced damage.

#J20 STEREOLOGY FREE#

A decrease in hippocampal adult neurogenesis has been described in these models and proposed to be a consequence of AОІ accumulation. Free Online Library: Age and Environment Influences on Mouse Prion Disease Progression: Behavioral Changes and Morphometry and Stereology of Hippocampal Astrocytes.(Research Article, Report) by 'Oxidative Medicine and Cellular Longevity' Biological sciences Aging Health aspects Aging (Biology) Animal behavior Analysis Immune response Observations Prion diseases Care and treatment Development. Shelanski, Michael L.ДЄPP overexpressing mice have been widely used in the study of Alzheimer's disease (AD), focusing mainly at older ages, with higher accumulation of amyloid-ОІ peptide (AОІ). Briefly, 3 coronal sections (30 m) at systematic random intervals through layer II of the entorhinal cortex were stained with cresyl violet, and the number of nuclei in a 46.3 Г— 31.5 Г— 6 m dissector were counted. Astroglial changes found in this study characterize an atrophic phenotype and a lost of glial support could explain the neuronal changes found at this age, although a primary neuronal alteration cannot be discarded.Increased Neurogenesis in Young Transgenic Mice Overexpressing Human APP _ For stereological analysis, sections were analyzed with the optical dissector method as described previously. Both cell types appear as especially vulnerable at early stages of the pathology. Our data support the idea of premature loss of both neurons and astrocytes in the hippocampus of AD mice at an age when there are no evidences of extensive amyloid pathology. These changes in adult-born neurons in the dentate gyrus correlated with a decrease in the expression of synaptophysin+ mossy fibers reaching CA3. Transgenic animals showed decreased numbers of DCX+ cells showing a mature as well as an immature morphology, although Tg animals had a relative shift towards a more mature morphology to the detriment of the immature population. We also analyzed the number and morphology of doublecortin+ (DCX) cells in the dentate gyrus to evaluate adult neurogenesis. Regarding neuronal changes, we found significantly less number of NeuN+ neurons in CA1 pyramidal layer of Tg mice but no changes were detected in dendritic spine density studied by means of a Golgi study. This study sought to evaluate Wnt signaling pathway interplay integrity across prefrontal lobe structures in AD patients compared to normal aging.

In Tg mice, we found a reduction in the number of GFAP+ astrocytes in CA1 region along with a decrease in astrocytic three-dimensional complexity studied as a surface/volume index, contrasting the established notion of a generalized astrogliosis accompanying the neurodegenerative process. Wnt pathway is involved in synaptic plasticity and neuronal survival, and alterations in Wnt signaling have previously been reported both in aging and neurodegenerative diseases, including AlzheimerвЂ™s disease (AD). Astrocytes were studied using glial fibrillary acidic protein (GFAP) immunofluorescence, confocal imaging and stereology. We evaluated transgenic (Tg) and control (NTg) mice at 5 months of age, when no amyloid plaques are present in the hippocampus and low cerebral levels of amyloid peptides are detectable. In the present study, using an animal model of AD, the transgenic PDAPP-J20 mouse (carrying human APP gene with Swe and Ind familial AD mutations), we studied early alterations in astroglia and neurons. Suspendisse massa odio, aliquet nec commodo et, venenatis ut velit. Nulla massa est, faucibus non semper quis, laoreet et sapien. Suspendisse dignissim bibendum lectus, quis ornare tortor elementum quis. In the context of AlzheimerВґs disease (AD), hippocampal alterations have been well described in advanced stages of the pathology, when widespread amyloid pathology is present and inflammatory changes and glial reactivity occur, but less attention has been directed to study early brain changes. Class aptent taciti sociosqu ad litora torquent per conubia nostra, per inceptos himenaeos.

J20 stereology